This study aimed to develop and evaluate health educational information system on internet to train health center workers. Internet-based Public Health Manpower Training Program was developed based on the findings of the needs of health center worker. It consisted of E-mail based on-line lesson. The effects of developed Internet-based Public Health Manpower Training Program were assessed by questionnaires of users about legibility, readability, satisfaction, changes in level of knowledge, and practical uses, etc. Most users(94.3%) of Internet-based Public Health Manpower Training Program reported that they were satisfied with the programs, and 90% of users reported that the programs improved their performance in health education, consultation and medical examination. Knowledge levels of users were elevated after program participation(statistically non significant). Internet-based Public Health Manpower Training Program had positive effects on the performance of health center workers, and we expect this program to help the planning for public health manpower.